TBR1 Monoclonal antibody

TBR1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

5C3A5

Cat No : 66564-1-Ig

Print datasheet

Synonyms

TBR-1, TBR 1, T-box brain protein 1, T brain 1, T box, brain, 1



经过测试的应用

Positive WB detected inpig brain tissue, pig cerebellum tissue, rat cerebellum tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy TBR1 Ready-To-Use IHC Kit. TBR1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66564-1-Ig targets TBR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsIHC, IF
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Immunogen TBR1 fusion protein Ag15262 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name T-box, brain, 1
Synonyms TBR-1, TBR 1, T-box brain protein 1, T brain 1, T box, brain, 1
Calculated Molecular Weight 682 aa, 74 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC104844
Gene Symbol TBR1
Gene ID (NCBI) 10716
RRIDAB_2881925
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16650
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBR1, also named T-box brain protein 1, is a 682 amino acid protein, which contains one T-box DNA-binding domain and localizes in the nucleus. TBR1 is expressed in the brain and as a transcriptional regulator is involved in developmental processes. TBR1 is required for normal brain development.

实验方案

Product Specific Protocols
WB protocol for TBR1 antibody 66564-1-IgDownload protocol
IHC protocol for TBR1 antibody 66564-1-IgDownload protocol
IF protocol for TBR1 antibody 66564-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Commun

LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis.

Authors - Huai-Bin Hu
mouseIHC

EMBO J

Centrosome defects cause microcephaly by activating the 53BP1-USP28-TP53 mitotic surveillance pathway.

Authors - Thao P Phan
mouseIF

Ann Neurol

Pathogenic MAST3 Variants in the STK Domain Are Associated with Epilepsy.

Authors - Egidio Spinelli
mouseIF

Biochem Biophys Res Commun

Neuron-specific loss of Ppp6c induces neonatal death and decreases the number of cortical neurons and interneurons

Authors - Miki Matsuoka
humanIHC

Nat Commun

iPSC-derived models of PACS1 syndrome reveal transcriptional and functional deficits in neuron activity

Authors - Lauren Rylaarsdam
ratIF

J Hazard Mater

Effects of nanoplastic exposure during pregnancy and lactation on neurodevelopment of rat offspring

Authors - Lei Tian