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  • Featured Product
  • KD/KO Validated

TDP-43 Recombinant antibody, PBS Only

TDP-43 Recombinant Antibody for WB,IP,Indirect ELISA,IF,FC

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB,IP,Indirect ELISA,IF,FC

Conjugate

Unconjugated

CloneNo.

16A22

Cat No : 80002-1-PBS

Print datasheet

Synonyms

ALS10, TAR DNA binding protein 43, TARDBP, tdp 43, TDP43, TDP-43

验证数据展示

  • WB analysis using 80002-1-RR (same clone as 80002-1-PBS)

    WB analysis using 80002-1-RR (same clone as 80002-1-PBS)

    Various cell lysates were subjected to SDS PAGE followed by western blot with 80002-1-RR (TDP-43 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

  • WB analysis of HAP1 using 80002-1-RR (same clone as 80002-1-PBS)

    WB analysis of HAP1 using 80002-1-RR (same clone as 80002-1-PBS)

    HAP1 (WT and TARDBP KO) lysates prepared with NP-40 buffer, 50 μg protein loaded. 80002-1-RR incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 80002-1-RR (same clone as 80002-1-PBS)

    WB analysis of HeLa using 80002-1-RR (same clone as 80002-1-PBS)

    WB result of TDP-43 antibody (80002-1-RR; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 transfected HeLa cells. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

  • IP experiment of HAP1 using 80002-1-RR (same clone as 80002-1-PBS)

    IP experiment of HAP1 using 80002-1-RR (same clone as 80002-1-PBS)

    HAP1 lysates prepared and IP of TARDBP performed using 2.0 μg of 80002-1-RR coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 80002-1-RR (same clone as 80002-1-PBS)

    IF Staining of rat brain using 80002-1-RR (same clone as 80002-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 80002-1-RR (TDP-43 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 80002-1-RR (same clone as 80002-1-PBS)

    IF Staining of SH-SY5Y using 80002-1-RR (same clone as 80002-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using TDP-43 antibody (80002-1-RR, Clone: 16A22 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staining with CoraLite555-Phalloidin. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 80002-1-RR (same clone as 80002-1-PBS)

    IF Staining of HeLa using 80002-1-RR (same clone as 80002-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 antibody (80002-1-RR, Clone: 16A22 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

  • IF Staining of HAP1 using 80002-1-RR (same clone as 80002-1-PBS)

    IF Staining of HAP1 using 80002-1-RR (same clone as 80002-1-PBS)

    HAP1 WT cells (yellow outline) and TARDBP KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 80002-1-RR at 1:200 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 80002-1-RR (same clone as 80002-1-PBS)

    FC experiment of HeLa using 80002-1-RR (same clone as 80002-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (for IF/FC) (80002-1-RR, Clone:16A22) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

产品信息

80002-1-PBS targets TDP-43 in WB, IP, IF, FC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,IP,Indirect ELISA,IF,FC
Tested Reactivity Human, mouse, rat
Immunogen TDP-43 fusion protein Ag1231 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name TAR DNA binding protein
Synonyms ALS10, TAR DNA binding protein 43, TARDBP, tdp 43, TDP43, TDP-43
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC001487
Gene Symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13148
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 80002-1-RR is a rabbit recombinant TDP-43 antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (PMID: 29581274)

80002-1-RR antibody works well in IF experiment.