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验证数据展示

  • WB analysis using 14199-1-AP

    WB analysis using 14199-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14199-1-AP (TNK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 14199-1-AP

    IHC staining of human prostate cancer using 14199-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14199-1-AP (TNK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TNK1 antibody included
  • IHC staining of human prostate cancer using 14199-1-AP

    IHC staining of human prostate cancer using 14199-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14199-1-AP (TNK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TNK1 antibody included

TNK1 Polyclonal antibody

TNK1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14199-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CD38 negative kinase 1, TNK1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HL-60 cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TNK1 Ready-To-Use IHC Kit. TNK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14199-1-AP targets TNK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 TNK1 fusion protein Ag5402 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tyrosine kinase, non-receptor, 1
别名 CD38 negative kinase 1, TNK1
计算分子量 72 kDa
观测分子量 72 kDa
GenBank蛋白编号BC035782
基因名称 TNK1
Gene ID (NCBI) 8711
RRIDAB_2205700
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13470
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TNK1 antibody 14199-1-APDownload protocol
IHC protocol for TNK1 antibody 14199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Immunol

Thirty-Eight-Negative Kinase 1 Is a Mediator of Acute Kidney Injury in Experimental and Clinical Traumatic Hemorrhagic Shock.

Authors - Rebecca Halbgebauer
View Article
human,ratWB, IHC

3 Biotech

Tyrosine kinase nonreceptor 1 (TNK1) knockdown ameliorates hemorrhage shock-induced kidney injury via inhibiting macrophage M1 polarization.

Authors - Miaolong Tang
View Article