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TNNI2 Polyclonal antibody

TNNI2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11634-1-AP

Print datasheet

Synonyms

AMCD2B, DA2B, FSSV, fsTnI, TNNI2, Troponin I fast skeletal muscle

验证数据展示

  • WB analysis using 11634-1-AP

    WB analysis using 11634-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skeletal muscle using 11634-1-AP

    WB analysis of mouse skeletal muscle using 11634-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 11634-1-AP

    IHC staining of mouse skeletal muscle using 11634-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11634-1-AP (TNNI2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 11634-1-AP

    IHC staining of mouse skeletal muscle using 11634-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11634-1-AP (TNNI2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, human kidney tissue, human spleen tissue, human skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11634-1-AP targets TNNI2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen TNNI2 fusion protein Ag2208 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name troponin I type 2 (skeletal, fast)
Synonyms AMCD2B, DA2B, FSSV, fsTnI, TNNI2, Troponin I fast skeletal muscle
Calculated Molecular Weight 182 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC032148
Gene Symbol TNNI2
Gene ID (NCBI) 7136
RRIDAB_2206109
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48788
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TNNI2 antibody 11634-1-APDownload protocol
IHC protocol for TNNI2 antibody 11634-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols