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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated TRAF6 Monoclonal antibody

TRAF6 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

FC (Intra) and More (1)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1D1E1

Cat No : CL647-66498

Print datasheet

Synonyms

RING finger protein 85, RNF85, TRAF6

验证数据展示

  • FC experiment of Jurkat using CL647-66498

    FC experiment of Jurkat using CL647-66498

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human TRAF6 (CL647-66498, Clone:1D1E1) (red), or 0.2 ug APC-65128 ; APC Mouse IgG2b Isotype Control (APC-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy TRAF6 Ready-To-Use IHC Kit. TRAF6 primary antibody included.

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

CL647-66498 targets TRAF6 in WB, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen TRAF6 fusion protein Ag24696 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TNF receptor-associated factor 6
Synonyms RING finger protein 85, RNF85, TRAF6
Calculated Molecular Weight 522 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC031052
Gene Symbol TRAF6
Gene ID (NCBI) 7189
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y4K3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 TRAF6 antibody CL647-66498Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Balkan Med J

Asiaticoside Down-Regulates HIF-1α to Inhibit Proliferation, Migration, and Angiogenesis in Thyroid Cancer Cells

Authors - Yi Zhang
View Article