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TRAPPC2 Polyclonal antibody

TRAPPC2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12484-1-AP

Print datasheet

Synonyms

hYP38334, MBP 1 interacting protein 2A, MIP 2A, SEDL, Sedlin, SEDLP, SEDT, TRAPPC2, TRAPPC2P1, TRS20, ZNF547L

验证数据展示

  • WB analysis of human brain using 12484-1-AP

    WB analysis of human brain using 12484-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 12484-1-AP

    IP experiment of PC-3 using 12484-1-AP

    IP result of anti-TRAPPC2 (IP:12484-1-AP, 3ug; Detection:12484-1-AP 1:500) with PC-3 cells lysate 2800ug.

经过测试的应用

Positive WB detected inhuman brain tissue, human liver tissue, human lung tissue, mouse brain tissue, mouse thymus tissue, PC-3 cells
Positive IP detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

12484-1-AP targets TRAPPC2 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Immunogen TRAPPC2 fusion protein Ag3201 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name trafficking protein particle complex 2
Synonyms hYP38334, MBP 1 interacting protein 2A, MIP 2A, SEDL, Sedlin, SEDLP, SEDT, TRAPPC2, TRAPPC2P1, TRS20, ZNF547L
Calculated Molecular Weight 140 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC016915
Gene Symbol TRAPPC2
Gene ID (NCBI) 6399
RRIDAB_2208136
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP0DI82
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAPPC2 is one component of the TRAPP multisubunit tethering complex involved in intracellular vesicle trafficking [PMID:21525244]. It acts as an adaptor for the TRAPP complex in mammalian cells, mediating interactions with both TRAPPC9 and TRAPPC8. It prevents transcriptional repression and induction of cell death by ENO1. Also it plays a role in vesicular transport from endoplasmic reticulum to Golgi.

实验方案

Product Specific Protocols
WB protocol for TRAPPC2 antibody 12484-1-APDownload protocol
IP protocol for TRAPPC2 antibody 12484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome.

Authors - Westlake Christopher J CJ
View Article
humanWB

J Biol Chem

The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.

Authors - Adrian Cuenca
View Article
ratWB

iScience

Mea6/cTAGE5 cooperates with TRAPPC12 to regulate PTN secretion and white matter development

Authors - Tiantian Ma
View Article