Tim23 Recombinant antibody

Tim23 Recombinant Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H16

Cat No : 82540-1-RR

Print datasheet

Synonyms

PRO1197, TIM23, TIMM23



经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, mouse heart tissue, rat heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82540-1-RR targets Tim23 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen Tim23 fusion protein Ag1620 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name translocase of inner mitochondrial membrane 23 homolog (yeast)
Synonyms PRO1197, TIM23, TIMM23
Calculated Molecular Weight 22 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC062707
Gene Symbol TIMM23
Gene ID (NCBI) 10431
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14925
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tim23, a key component of the mitochondrial preprotein translocase, is anchored in the inner membrane by its C-terminal domain and exposes an intermediate domain in the intermembrane space that functions as a presequence receptor. Tim23 is the main component of the translocation channel of the TIM23 complex. (PMID: 10830167, PMID: 25083920)

实验方案

Product Specific Protocols
WB protocol for Tim23 antibody 82540-1-RRDownload protocol
IHC protocol for Tim23 antibody 82540-1-RRDownload protocol
IF protocol for Tim23 antibody 82540-1-RRDownload protocol
IP protocol for Tim23 antibody 82540-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols