CoraLite®594-conjugated USP11 Monoclonal antibody

USP11 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2G6E7

Cat No : CL594-67805

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Synonyms

Deubiquitinating enzyme 11, Ubiquitin thiolesterase 11, UHX1, USP11



经过测试的应用

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67805-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67805 targets USP11 in IF applications and shows reactivity with Human samples.

Tested Applications IF/ICC
Tested Reactivity Human
Immunogen USP11 fusion protein Ag19058 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin specific peptidase 11
Synonyms Deubiquitinating enzyme 11, Ubiquitin thiolesterase 11, UHX1, USP11
Calculated Molecular Weight 963 aa, 110 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC000350
Gene Symbol USP11
Gene ID (NCBI) 8237
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP51784
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.

实验方案

Product Specific Protocols
IF protocol for CL594 USP11 antibody CL594-67805Download protocol
Standard Protocols
Click here to view our Standard Protocols