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Unc119b Polyclonal antibody

Unc119b Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Canine

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26201-1-AP

Print datasheet

Synonyms

AA407659, AI414892, unc 119 homolog B (C. elegans), Unc119b

验证数据展示

  • WB analysis using 26201-1-AP

    WB analysis using 26201-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26201-1-AP (Unc119b antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of MDCK using 26201-1-AP

    IF Staining of MDCK using 26201-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 26201-1-AP (Unc119b antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, MDCK cells, mouse kidney tissue, HEK-293 cells, HeLa cells
Positive IF detected inMDCK cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26201-1-AP is Unc119b Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human, mouse, Canine
Immunogen Unc119b fusion protein Ag24599 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name unc-119 homolog B (C. elegans)
Synonyms AA407659, AI414892, unc 119 homolog B (C. elegans), Unc119b
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-31 kDa
GenBank Accession NumberBC008617
Gene Symbol Unc119b
Gene ID (NCBI) 106840
RRIDAB_2880422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8C4B4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mouse Unc119b is related closely to Unc119, a homolog of C. elegans unc119 protein which was first discovered in C. elegans on the basis of a spontaneous mutation affecting locomotion, feeding behavior and chemosensation. Mouse Unc119b shares 90% sequence identity with human UNC119b protein. UNC119b is a myristoyl-binding protein that acts as a cargo adapter: specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. Myristoyl-binding activity of UNC119b is required to localize NPHP3 to the primary cilium (PMID: 22085962).

实验方案

Product Specific Protocols
WB protocol for Unc119b antibody 26201-1-APDownload protocol
IF protocol for Unc119b antibody 26201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols