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  • Featured Product
  • KD/KO Validated

Vimentin Monoclonal antibody, PBS Only (Detector)

Vimentin Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

3H9D1

Cat No : 60330-1-PBS

Print datasheet

Synonyms

VIM, Vimentin

验证数据展示

  • IHC staining of human breast cancer using 60330-1-Ig (same clone as 60330-1-PBS)

    IHC staining of human breast cancer using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 60330-1-Ig (same clone as 60330-1-PBS)

    IHC staining of human colon using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:102400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60330-1-Ig (same clone as 60330-1-PBS)

    IHC staining of human liver cancer using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat cells using 60330-1-Ig (same clone as 60330-1-PBS)

    WB analysis of Jurkat cells using 60330-1-Ig (same clone as 60330-1-PBS)

    WB result of Vimentin antibody (60330-1-Ig, 1:4000) with si-Control and si-Vimentin transfected Jurkat cells. This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 60330-1-Ig (same clone as 60330-1-PBS)

    IHC staining of human appendicitis using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60330-1-Ig (same clone as 60330-1-PBS)

    IF Staining of HepG2 using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60330-1-Ig (Vimentin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • WB analysis using 60330-1-Ig (same clone as 60330-1-PBS)

    WB analysis using 60330-1-Ig (same clone as 60330-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60330-1-Ig (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 60330-1-Ig (same clone as 60330-1-PBS)

    FC experiment of Jurkat using 60330-1-Ig (same clone as 60330-1-PBS)

    1X10^6 Jurkat cells were stained with 0.2 ug Anti-Human Vimentin (60330-1-Ig, Clone:3H9D1) and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1500 (red), or stained with 0.2 ug isotype control and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1500 (blue). Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 60330-1-Ig (same clone as 60330-1-PBS)

    IF Staining of HUVEC using 60330-1-Ig (same clone as 60330-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Vimentin antibody (60330-1-Ig, Clone: 3H9D1 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

  • Standard curve of MP50158-1

    Standard curve of MP50158-1

    Cytometric bead array standard curve of MP50158-1, Vimentin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60330-2-PBS. Detection antibody: 60330-1-PBS. Standard:ag0489. Range: 3.125-100 ng/mL.

产品信息

60330-1-PBS targets Vimentin as part of a matched antibody pair:

MP50158-1: 60330-2-PBS capture and 60330-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF, FC, IHC, Indirect ELISA, Cytometric bead array
Tested Reactivity human, mouse, rat
Immunogen Vimentin fusion protein Ag0489 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vimentin
Synonyms VIM, Vimentin
Calculated Molecular Weight 466 aa, 54 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC000163
Gene Symbol VIM
Gene ID (NCBI) 7431
ENSEMBL Gene IDENSG00000026025
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08670
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.