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  • Featured Product
  • KD/KO Validated

Vimentin Recombinant antibody, PBS Only

Vimentin Recombinant Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

6K21

Cat No : 80232-1-PBS

Print datasheet

Synonyms

Cell and organelle markers, Cytoskeleton Marker, FLJ36605, VIM, vimentin

验证数据展示

  • WB analysis using 80232-1-RR (same clone as 80232-1-PBS)

    WB analysis using 80232-1-RR (same clone as 80232-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80232-1-RR (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 80232-1-RR (same clone as 80232-1-PBS)

    WB analysis of A549 using 80232-1-RR (same clone as 80232-1-PBS)

    WB result of Vimentin antibody (80232-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Vimentin transfected HeLa cells. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 80232-1-RR (same clone as 80232-1-PBS)

    IHC staining of human breast cancer using 80232-1-RR (same clone as 80232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 80232-1-RR (same clone as 80232-1-PBS)

    IF Staining of HepG2 using 80232-1-RR (same clone as 80232-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Vimentin antibody (80232-1-RR, Clone: 6K21 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

产品信息

80232-1-PBS targets Vimentin in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF
Tested Reactivity Human, mouse, rat
Immunogen Vimentin fusion protein Ag0489 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name vimentin
Synonyms Cell and organelle markers, Cytoskeleton Marker, FLJ36605, VIM, vimentin
Calculated Molecular Weight 466 aa, 54 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC000163
Gene Symbol VIM
Gene ID (NCBI) 7431
ENSEMBL Gene IDENSG00000026025
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08670
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).