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CoraLite® Plus 488-conjugated Vinculin Polyclonal antibody

Vinculin Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-26520

Print datasheet

Synonyms

CMD1W, Metavinculin, MVCL, VCL, vinculin

验证数据展示

  • IF Staining of HepG2 using CL488-26520

    IF Staining of HepG2 using CL488-26520

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Vinculin antibody (CL488-26520) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HepG2 using CL488-26520

    FC experiment of HepG2 using CL488-26520

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Vinculin (CL488-26520) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells, A549 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 26520-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26520 targets Vinculin in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse
Immunogen Vinculin fusion protein Ag24942 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vinculin
Synonyms CMD1W, Metavinculin, MVCL, VCL, vinculin
Calculated Molecular Weight 1133 aa, 124 kDa
Observed Molecular Weight 117 kDa
GenBank Accession NumberBC039174
Gene Symbol VCL
Gene ID (NCBI) 7414
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18206
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Vinculin antibody CL488-26520Download protocol
FC protocol for CL Plus 488 Vinculin antibody CL488-26520Download protocol
Standard Protocols
Click here to view our Standard Protocols