• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

WWTR1 Monoclonal antibody, PBS Only

WWTR1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2A12A10

Cat No : 66500-1-PBS

Print datasheet

Synonyms

TAZ, WWTR1

验证数据展示

  • WB analysis using 66500-1-Ig (same clone as 66500-1-PBS)

    WB analysis using 66500-1-Ig (same clone as 66500-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66500-1-Ig (same clone as 66500-1-PBS)

    WB analysis of HepG2 using 66500-1-Ig (same clone as 66500-1-PBS)

    WB result of TAZ antibody (66500-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAZ transfected HepG2 cells. This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

    IHC staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

    IHC staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

    IF Staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TAZ antibody (66500-1-Ig, Clone: 2A12A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

产品信息

66500-1-PBS targets WWTR1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF
Tested Reactivity Human, rat, mouse
Immunogen WWTR1 fusion protein Ag13330 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name WW domain containing transcription regulator 1
Synonyms TAZ, WWTR1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC014052
Gene Symbol WWTR1
Gene ID (NCBI) 25937
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9GZV5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.