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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated WWTR1 Polyclonal antibody

WWTR1 Polyclonal Antibody for IF/ICC,FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC,FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-23306

Print datasheet

Synonyms

TAZ, WWTR1

验证数据展示

  • IF Staining of U-251 using CL488-23306

    IF Staining of U-251 using CL488-23306

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CoraLite® Plus 488 WWTR1 antibody (CL488-23306) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-23306

    FC experiment of HeLa using CL488-23306

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human WWTR1 (CL488-23306)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inU-251 cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 23306-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-23306 targets WWTR1 in IF, FC (Intra) applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC,FC (Intra)
Tested Reactivity human, rat
Immunogen WWTR1 fusion protein Ag19659 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WW domain containing transcription regulator 1
Synonyms TAZ, WWTR1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC014052
Gene Symbol WWTR1
Gene ID (NCBI) 25937
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZV5
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 WWTR1 antibody CL488-23306Download protocol
FC protocol for CL Plus 488 WWTR1 antibody CL488-23306Download protocol
Standard Protocols
Click here to view our Standard Protocols