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XIAP Polyclonal antibody

XIAP Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10037-1-Ig

Print datasheet

Synonyms

API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, X linked IAP, XIAP, XLP2



经过测试的应用

Positive WB detected inRAW 264.7 cells, Jurkat cells, HepG2 cells, COLO 320 cells, HeLa cells, C6 cells, NIH/3T3 cells, Raji cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10037-1-Ig targets XIAP in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications IHC, WB, ELISA
Cited Applications IHC, WB
Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse
Immunogen XIAP fusion protein Ag0022 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name X-linked inhibitor of apoptosis
Synonyms API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, X linked IAP, XIAP, XLP2
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 45-57 kDa
GenBank Accession NumberBC032729
Gene Symbol XIAP
Gene ID (NCBI) 331
RRIDAB_2215009
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP98170
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. The MW of XIAP is 45-56 kDa.

实验方案

Product Specific Protocols
WB protocol for XIAP antibody 10037-1-IgDownload protocol
IHC protocol for XIAP antibody 10037-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer.

Authors - Kai Yuan
humanWB

Cancer Res

DAB2IP is a bifunctional tumor suppressor that regulates wildtype RAS and inflammatory cascades in KRAS mutant colon cancer

Authors - Abigail L Miller
humanIHC

Cancer Res

An ATM/TRIM37/NEMO axis counteracts genotoxicity by activating nuclear-to-cytoplasmic NF-κB signaling.

Authors - Geyan Wu
humanWB

Acta Pharmacol Sin

Aspirin modulates succinylation of PGAM1K99 to restrict the glycolysis through NF-κB/HAT1/PGAM1 signaling in liver cancer.

Authors - Yu-Fei Wang
humanWB

Int J Nanomedicine

Synergism of cisplatin-oleanolic acid co-loaded calcium carbonate nanoparticles on hepatocellular carcinoma cells for enhanced apoptosis and reduced hepatotoxicity.

Authors - Muhammad Waseem Khan
humanWB

Oncotarget

CRNDE affects the malignant biological characteristics of human glioma stem cells by negatively regulating miR-186.

Authors - Jian Zheng