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验证数据展示

  • WB analysis using 11267-1-AP

    WB analysis using 11267-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 11267-1-AP (XRN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

XRN2 Polyclonal antibody

XRN2 Polyclonal Antibody for WB, ELISA
Cat No. 11267-1-AP

产品说明书

引用文献(8)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, ELISA and More (5)

别名

5 3 exoribonuclease 2, 5' 3' exoribonuclease 2, DHM1 like protein, DHP protein, XRN2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, COLO 320 cells, HEK-293 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy XRN2 Ready-To-Use IHC Kit. XRN2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11267-1-AP targets XRN2 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IP, IF, IHC, CoIP, ChIP
经测试反应性 human
文献引用反应性human, mouse
免疫原 XRN2 fusion protein Ag1788 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 5'-3' exoribonuclease 2
别名 5 3 exoribonuclease 2, 5' 3' exoribonuclease 2, DHM1 like protein, DHP protein, XRN2
计算分子量 104 kDa
观测分子量 104 kDa
GenBank蛋白编号BC006417
基因名称 XRN2
Gene ID (NCBI) 22803
RRIDAB_2288787
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H0D6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XRN2 is one exonuclease that degrades the Pol II associated product of poly(A) site cleavage, which is crucial for Pol II termination. During transcription termination, XRN2 cleavages at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.

实验方案

Product Specific Protocols
WB protocol for XRN2 antibody 11267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article
humanWB

Cell

Microprocessor, Setx, Xrn2, and Rrp6 co-operate to induce premature termination of transcription by RNAPII.

Authors - Wagschal Alexandre A
  • KD Validated
View Article
humanChIP,CoIP,WB

EMBO J

Arginine methylation of the DDX5 helicase RGG/RG motif by PRMT5 regulates resolution of RNA:DNA hybrids.

Authors - Sofiane Y Mersaoui
  • KD Validated
View Article

J Cell Biol

Intronless mRNAs transit through nuclear speckles to gain export competence.

Authors - Ke Wang
View Article
humanWB

PLoS Pathog

Characterization of miR-122-independent propagation of HCV.

Authors - Chikako Ono
  • KD Validated
View Article
humanWB, IF

J Virol

Dissecting the roles of the 5' exoribonucleases xrn1 and xrn2 in restricting hepatitis C virus replication.

Authors - You Li
  • KD Validated
View Article