CoraLite® Plus 488-conjugated CD20 Monoclonal antibody
CD20 Monoclonal Antibody for IF/ICC,IF-P
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
IF/ICC,IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4A7G3
验证数据展示
经过测试的应用
Positive IF detected in | human tonsillitis tissue, human lung cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60271-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-60271 targets CD20 in IF applications and shows reactivity with human samples.
Tested Applications | IF/ICC,IF-P |
Tested Reactivity | human |
Immunogen | Recombinant Protein 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | membrane-spanning 4-domains, subfamily A, member 1 |
Synonyms | CD20, MS4A1 |
Calculated Molecular Weight | 297 aa, 33 kDa |
GenBank Accession Number | BC002807 |
Gene Symbol | MS4A1 |
Gene ID (NCBI) | 931 |
RRID | AB_2883132 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P11836 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells. This antibody is conjugated with CL488(Ex/Em 488 nm/515 nm).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 CD20 antibody CL488-60271 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |