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验证数据展示

  • WB analysis using 24046-1-AP

    WB analysis using 24046-1-AP

    Mouse liver tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 24046-1-AP

    IHC staining of human testis using 24046-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of SH-SY5Y using 24046-1-AP

    IF Staining of SH-SY5Y using 24046-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 24046-1-AP (ZDHHC9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver using 24046-1-AP

    IHC staining of human liver using 24046-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24046-1-AP (ZDHHC9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ZDHHC9 Polyclonal antibody

ZDHHC9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24046-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

EC:2.3.1.225, DHHC-9, DHHC9, DHHC 9, CXorf11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated

此产品已下架，相关产品可参考CatNo. 82739-1-RR
Our concentration is detected with BSA as standard. A higher concentration will be obtained with IgG as standard.

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经过测试的应用

Positive WB detected inmouse liver tissue, SH-SY5Y cells, mouse skeletal muscle tissue, mouse brain tissue
Positive IHC detected inhuman testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24046-1-AP targets ZDHHC9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 ZDHHC9 fusion protein Ag20881 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger, DHHC-type containing 9
别名 EC:2.3.1.225, DHHC-9, DHHC9, DHHC 9, CXorf11
计算分子量 41 kDa
观测分子量 38-48 kDa
GenBank蛋白编号BC006200
基因名称 ZDHHC9
Gene ID (NCBI) 51114
RRIDAB_2879415
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9Y397
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZDHHC9, also named as CXorf11, ZDHHC10, ZNF379 or ZNF380, is a 364 amino acid protein, which contains one DHHC-type zinc finger and belongs to the DHHC palmitoyltransferase activity. ZDHHC9 localizes in the endoplasmic reticulum membrane and is highly expressed in kidney, skeletal muscle, brain, lung and liver. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.

实验方案

Product Specific Protocols
WB protocol for ZDHHC9 antibody 24046-1-APDownload protocol
IHC protocol for ZDHHC9 antibody 24046-1-APDownload protocol
IF protocol for ZDHHC9 antibody 24046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIHC,IF

Discov Oncol

ZDHHC9: a promising therapeutic target for triple-negative breast cancer through immune modulation and immune checkpoint blockade resistance

Authors - Chao Xu
View Article
humanWB

Cancer Lett

Palmitoylation alters LDHA activity and pancreatic cancer response to chemotherapy

Authors - Luojun Chen
View Article
mouseWB,IF

Adv Mater

Multifunctional Copper-Phenolic Nanopills Achieve Comprehensive Polyamines Depletion to Provoke Enhanced Pyroptosis and Cuproptosis for Cancer Immunotherapy

Authors - Guoqing Zhu
View Article