验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, SH-SY5Y cells, mouse skeletal muscle tissue, mouse brain tissue |
| Positive IHC detected in | human testis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24046-1-AP targets ZDHHC9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | ZDHHC9 fusion protein Ag20881 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | zinc finger, DHHC-type containing 9 |
| Synonyms | EC:2.3.1.225, DHHC-9, DHHC9, DHHC 9, CXorf11 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 38-48 kDa |
| GenBank Accession Number | BC006200 |
| Gene Symbol | ZDHHC9 |
| Gene ID (NCBI) | 51114 |
| RRID | AB_2879415 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q9Y397 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZDHHC9, also named as CXorf11, ZDHHC10, ZNF379 or ZNF380, is a 364 amino acid protein, which contains one DHHC-type zinc finger and belongs to the DHHC palmitoyltransferase activity. ZDHHC9 localizes in the endoplasmic reticulum membrane and is highly expressed in kidney, skeletal muscle, brain, lung and liver. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZDHHC9 antibody 24046-1-AP | Download protocol |
| IHC protocol for ZDHHC9 antibody 24046-1-AP | Download protocol |
| IF protocol for ZDHHC9 antibody 24046-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Discov Oncol ZDHHC9: a promising therapeutic target for triple-negative breast cancer through immune modulation and immune checkpoint blockade resistance | ||
Cancer Lett Palmitoylation alters LDHA activity and pancreatic cancer response to chemotherapy | ||
Adv Mater Multifunctional Copper-Phenolic Nanopills Achieve Comprehensive Polyamines Depletion to Provoke Enhanced Pyroptosis and Cuproptosis for Cancer Immunotherapy |
