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ZHX2 Monoclonal antibody

ZHX2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4E12F2

Cat No : 68268-1-Ig

Print datasheet

Synonyms

AFP regulator 1, AFR1, Alpha fetoprotein regulator 1, KIAA0854, RAF, Regulator of AFP, ZHX2, zinc fingers and homeoboxes 2

验证数据展示

  • WB analysis using 68268-1-Ig

    WB analysis using 68268-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68268-1-Ig (ZHX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HEK-293 using 68268-1-Ig

    WB analysis of HEK-293 using 68268-1-Ig

    WB result of ZHX2 antibody (68268-1-Ig; 1:24000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZHX2 transfected HEK-293 cells.

经过测试的应用

Positive WB detected inSH-SY5Y cells, HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy ZHX2 Ready-To-Use IHC Kit. ZHX2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68268-1-Ig targets ZHX2 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Applications WB, ELISA
Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Immunogen ZHX2 fusion protein Ag14420 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name zinc fingers and homeoboxes 2
Synonyms AFP regulator 1, AFR1, Alpha fetoprotein regulator 1, KIAA0854, RAF, Regulator of AFP, ZHX2, zinc fingers and homeoboxes 2
Calculated Molecular Weight 837 aa, 92 kDa
Observed Molecular Weight92-100 kDa
GenBank Accession NumberBC042145
Gene Symbol ZHX2
Gene ID (NCBI) 22882
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y6X8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc fingers and homeoboxes protein 2 is a protein that in humans is encoded by the ZHX2 gene. ZHX2 Act as a transcriptional repressor, represses the promoter activity of the CDC25C gene stimulated by NFYA (PMID:12741956). In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.

实验方案

Product Specific Protocols
WB protocol for ZHX2 antibody 68268-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols