Beta Actin Recombinant antibody
Beta Actin Recombinant Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, pig, rat, mouse and More (3)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
253
CloneNo.
4H1
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, rat brain tissue, mouse brain tissue, pig brain tissue |
Positive IHC detected in | rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | U2OS cells, A549 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:950-1:3800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
81115-1-RR targets Beta Actin in WB, IF, IHC, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF |
Tested Reactivity | Human, pig, rat, mouse |
Cited Reactivity | human, mouse, rat, chicken, monkey, pig |
Immunogen | Beta Actin fusion protein Ag14521 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | actin, beta |
Synonyms | ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin |
Calculated Molecular Weight | 375 aa, 42 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | BC002409 |
Gene Symbol | ACTB |
Gene ID (NCBI) | 60 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P60709 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Beta Actin antibody 81115-1-RR | Download protocol |
IHC protocol for Beta Actin antibody 81115-1-RR | Download protocol |
IF protocol for Beta Actin antibody 81115-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Adv Mater Ultrasound Imaging of Tumor Vascular CD93 with MMRN2 Modified Microbubbles for Immune Microenvironment Prediction | ||
ACS Nano Long Noncoding RNA URB1-Antisense RNA 1 (AS1) Suppresses Sorafenib-Induced Ferroptosis in Hepatocellular Carcinoma by Driving Ferritin Phase Separation | ||
J Med Virol Differential pathogenic and molecular features in neurological infection of SARS-CoV-2 Omicron BA.5.2 and BA.2.75 and Delta | ||
J Med Virol SARS-CoV-2 nucleocapsid protein enhances the level of mitochondrial reactive oxygen species | ||
Research (Wash D C) Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis | ||
J Control Release Sertoli cell-derived extracellular vesicles traverse the blood-testis barrier and deliver miR-24-3p inhibitor into germ cells improving sperm mobility |