验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue, mouse colon tissue, rat lung tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28035-1-AP targets IL-33 in WB, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | mouse, rat |
| Immunogen | IL-33 fusion protein Ag27846 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | interleukin 33 |
| Synonyms | 9230117N10Rik, Il 33, Il1f11, Il33, IL-33, interleukin 33, NF HEV |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | NM_001164724 |
| Gene Symbol | IL-33 |
| Gene ID (NCBI) | 77125 |
| RRID | AB_2881042 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8BVZ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Interleukin-33 (IL-33) is a tissue-derived nuclear cytokine from the IL-1 family abundantly expressed in endothelial cells, epithelial cells and fibroblast-like cells, both during homeostasis and inflammation. IL-33 activates many immune cell types involved in type-2 immunity and allergic inflammation, including ILC2s, mast cells, Th2 cells, eosinophils, basophils, dendritic cells and alternatively activated macrophages (AAM). As a cytokine, IL-33 interacts with the receptors ST2 (also known as IL1RL1) and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. IL-33 is also effective in reversing Alzheimer-like symptoms in APP/PS1 mice, by reversing the buildup and preventing the new formation of amyloid plaques. IL-33 is synthesized as a 30-34 kD full-length form and 20 kDa form mature form.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-33 antibody 28035-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
