ki67 Polyclonal antibody
ki67 Polyclonal Antibody for IF/ICC,IF-Fro,IF-P, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse and More (2)
Applications
IF/ICC,IF-Fro,IF-P, FC, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | NIH/3T3 cells, mouse colon tissue, mouse spleen tissue |
Positive FC detected in | NIH/3T3 cells |
推荐稀释比
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28074-1-AP targets ki67 in WB, IF, FC, IHC, ELISA, Cell treatment applications and shows reactivity with mouse samples.
Tested Applications | IF/ICC,IF-Fro,IF-P, FC, IHC, ELISA |
Cited Applications | WB, IF, IHC, Cell treatment |
Tested Reactivity | mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | ki67 fusion protein Ag27894 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | antigen identified by monoclonal antibody Ki 67 |
Synonyms | D630048A14Rik, Ki 67, Ki67, Mki67 |
Calculated Molecular Weight | 351 kDa |
GenBank Accession Number | NM_001081117 |
Gene Symbol | Mki67 |
Gene ID (NCBI) | 17345 |
RRID | AB_2918145 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | E9PVX6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IHC protocol for ki67 antibody 28074-1-AP | Download protocol |
IF protocol for ki67 antibody 28074-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Biosens Bioelectron 3D co-culture of macrophages and fibroblasts in a sessile drop array for unveiling the role of macrophages in skin wound-healing | ||
Cancer Lett Icaritin with autophagy/mitophagy inhibitors synergistically enhances anticancer efficacy and apoptotic effects through PINK1/Parkin-mediated mitophagy in hepatocellular carcinoma | ||
Phytomedicine Kaempferol inhibits non-homologous end joining repair via regulating Ku80 stability in glioma cancer | ||
Biochem Pharmacol Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT | ||
Genes Cells Local anesthetic lidocaine induces growth suppression of HeLa cells by decreasing and changing the cellular localization of the proliferation marker Ki-67 | ||
J Oncol EGCG Enhances the Chemosensitivity of Colorectal Cancer to Irinotecan through GRP78-MediatedEndoplasmic Reticulum Stress |