Nestin Polyclonal antibody
Nestin Polyclonal Antibody for FC, IF/ICC,IF-P, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, rat, mouse and More (1)
Applications
FC, IF/ICC,IF-P, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | SGC-7901 cells, U-251 cells, U-87 MG cells |
Positive IF detected in | rat brain tissue, mouse kidney tissue, human kidney tissue, U-251 cells |
Positive FC detected in | SH-SY5Y cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
29285-1-AP targets Nestin in WB, IF, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.
Tested Applications | FC, IF/ICC,IF-P, WB, ELISA |
Cited Applications | IF |
Tested Reactivity | Human, rat, mouse |
Cited Reactivity | human |
Immunogen | Nestin fusion protein Ag29865 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | nestin |
Synonyms | NES, nestin |
Calculated Molecular Weight | 177 kDa |
Observed Molecular Weight | 240-280 kDa |
GenBank Accession Number | NM_006617 |
Gene Symbol | NES |
Gene ID (NCBI) | 10763 |
RRID | AB_2918268 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P48681 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Nestin antibody 29285-1-AP | Download protocol |
IF protocol for Nestin antibody 29285-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Bioact Mater Construction of functional neural network tissue combining CBD-NT3-modified linear-ordered collagen scaffold and TrkC-modified iPSC-derived neural stem cells for spinal cord injury repair |