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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human Cystatin C protein- GMP grade

EC50

0.5-2.6 µg/mL

Species Reactivity

human

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat No : HZ-1211-GMP

Print datasheet

Synonyms

ARMD11, CST3, Cystatin 3, cystatin C, Gamma trace, Post gamma globulin

Validation Data Gallery

  • Purity of GMP recombinant human Cystatin C was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of GMP recombinant human Cystatin C was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant Human GMP Cystatin C (HZ-1211-GMP) inhibits papain protease activity in a dose-dependent manner. Papain protease activity was measured by colorimetric assay using L-BAPA as the substrate. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range form 0.5-2.6 µg/mL.

    Recombinant Human GMP Cystatin C (HZ-1211-GMP) inhibits papain protease activity in a dose-dependent manner. Papain protease activity was measured by colorimetric assay using L-BAPA as the substrate. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range form 0.5-2.6 µg/mL.

Technical Specifications

GeneID 1471
Species Human
Expression HEK293
EC50 0.5-2.6 µg/mL
Purity >95%
Endotoxin<0.1 EU/μg
Accession NumberP01034
Molecular Mass 15 to 18 kDa reduced, 14 to 17 kDa non-reduced, monomer, non-glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4. Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Cystatin C is a 13-kDa protein that is expressed globally in the body. In healthy individuals, glomerular filtration in the kidney maintains it at a safe level. When kidney function is impaired, Cystatin C levels rise quickly. This makes it an early and sensitive biomarker of renal dysfunction (PMID 24848523). A mutation in Cystatin C has been associated with amyloid angiopathy (PMID 11409420). Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus.

Synonyms

ARMD11, CST3, Cystatin 3, cystatin C, Gamma trace, Post gamma globulin