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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human DKK-1 protein

Activity

7-70 ng/mL

Species Reactivity

human

Purity

>95%

Cat No : HZ-1314

Print datasheet

Synonyms

SK, Dickkopf-1, Dickkopf-related protein-1, mDkk-1

Validation Data Gallery

  • Purity of recombinant human DKK-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human DKK-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human DKK-1 (HZ-1314) inhibits dose-dependent luciferase production by Wnt3A in a HEK293 reporter cell line. Luciferase assay production was assessed by One-Step ™ luciferase assay kit. Hek293 cells were treated with increasing amounts of DKK-1 as well as 150ng/mL of recombinant Wnt3A (HZ-1296) for 6 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 7-70ng/mL.

    Recombinant human DKK-1 (HZ-1314) inhibits dose-dependent luciferase production by Wnt3A in a HEK293 reporter cell line. Luciferase assay production was assessed by One-Step ™ luciferase assay kit. Hek293 cells were treated with increasing amounts of DKK-1 as well as 150ng/mL of recombinant Wnt3A (HZ-1296) for 6 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 7-70ng/mL.

Technical Specifications

GeneID 22943
Species Human
Expression HEK293
Activity 7-70 ng/mL
Purity >95%
Endotoxin<1 EU/μg
Accession NumberO94907
Molecular Mass 37-51 kDa reduced, 35-49 non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

DKK-1 (Dickkopf WNT signaling pathway inhibitor 1) is a member of the DKK protein family. Other members of this family include DKK-2, DKK-3, and DKK-4. The DKK family proteins share 35-40 % homology between various members. Recombinant human DKK-1 expressed in HEK293 cells is a 35-45kDa glycosylated protein. It is a secreted protein that functions as a negative regulator of Wnt/β-catenin signaling pathway. Wnt/ β-catenin signaling pathway involves binding of the Wnt ligands to the seven-transmembrane receptor, Frizzled, and the coreceptor lipoprotein-related protein 5 and 6 (LRP5/6). DKK1 forms a ternary complex with LRP5/6 and another receptor, Kremen, followed by endocytosis of this complex and removal of LRP5/6 from the cell surface. It has been suggested that DKK-1 permits anterior development by inhibiting Wnt/ β-catenin signaling, which is essential for posterior patterning in vertebrates. The balance between Wnt signaling and DKK-1 inhibition is critical for bone formation and homeostasis and insufficient or excess DKK-1 activity in bone results in increased or decreased bone density, respectively. (PMID: 17143291; 12050670; 10570958)

Synonyms

SK, Dickkopf-1, Dickkopf-related protein-1, mDkk-1

Publications

SpeciesTitle
human

Cell Stem Cell

Efficient Generation of CA3 Neurons from Human Pluripotent Stem Cells Enables Modeling of Hippocampal Connectivity In Vitro.

Authors - Anindita Sarkar
View Article