• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human EPO protein- GMP grade

EC50

0.2-3.0 ng/mL

Specific Activity

minimally 3.0 x 105 IU/mg, typically 3.5 x 105 IU/mg

Species Reactivity

human

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat No : HZ-1168-GMP

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Synonyms

EP, EPO, Epoetin, erythropoietin, MVCD2



Technical Specifications

GeneID 2056
Species Human
Expression HEK293
EC50 0.2-3.0 ng/mL
Specific Activityminimally 3.0 x 105 IU/mg, typically 3.5 x 105 IU/mg
Purity >95%
Endotoxin< 0.1 EU/µg
Accession NumberP01588
Molecular Mass 36 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Erythropoietin (Epo) is a member of the EPO/TPO family. It is a secreted, glycosylated cytokine composed of four alpha helical bundles. Epo is found in the plasma and regulates erythrocyte production by promoting erythroid differentiation and initiating hemoglobin synthesis through activation of its cognate receptor (PMID 29458727). Epo also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types (PMID 11854521).

Synonyms

EP, EPO, Epoetin, erythropoietin, MVCD2