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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FGF-1 protein

Activity

0.5-2.5 ng/mL

Species Reactivity

human

Purity

> 95%

Cat No : HZ-1327

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Synonyms

Acidic Fibroblast Growth Factor 1, Heparin-Binding growth Factor 1, FGF-acidic, ECGF-beta, HBGF-1, FGF-1, Fibroblast Growth Factor Acidic

Validation Data Gallery

  • Purity of recombinant human FGF-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human FGF-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human FGF-1 (HZ-1327) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGF-1 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.5-2.5 ng/mL.

    Recombinant human FGF-1 (HZ-1327) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGF-1 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.5-2.5 ng/mL.

  • Proteintech FGF-1 demonstrates greater induction of proliferation and equivalent EC50 to leading competitors. Recombinant human FGF-1 (HZ-1327) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGFbasic-TS from either a leading competitor or Proteintech for 48 hours.

    Proteintech FGF-1 demonstrates greater induction of proliferation and equivalent EC50 to leading competitors. Recombinant human FGF-1 (HZ-1327) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGFbasic-TS from either a leading competitor or Proteintech for 48 hours.

Technical Specifications

GeneID 2246
Species Human
Expression HEK293
Activity 0.5-2.5 ng/mL
Purity > 95%
Endotoxin<1 EU/µg
Accession NumberC9JDC5
Molecular Mass 18 kDa reduced, 16 kDa non-reduced, monomer, non-glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

FGF-1 is a non-glycosylated and single polypeptide protein that is a well-classified member of the fibroblast growth factor (FGF) superfamily. It stimulates DNA synthesis and proliferation in a wide range of cells and is also involved in promoting angiogenesis and wound healing processes. It is capable of binding with all four FGFRs and exerts its intracellular effects through downstream PLCγ and MAPK pathways. Dysregulated FGF-1 signaling has been associated with tumor invasion and metastasis. It is also capable of inducing apoptosis in response to vascular injury and may have use as a potential therapeutic for cardiovascular diseases (PMID: 15469994, 24829797).

Synonyms

Acidic Fibroblast Growth Factor 1, Heparin-Binding growth Factor 1, FGF-acidic, ECGF-beta, HBGF-1, FGF-1, Fibroblast Growth Factor Acidic