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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-21 protein

Activity

0.25-1.25 ng/mL

Species Reactivity

human

Purity

>95%

Cat No : HZ-1319

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Synonyms

Interleukin 21, Interleukin-21, Interleukin-21 isoform, Za11, CVID11

Validation Data Gallery

  • Purity of recombinant human IL-21 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-21 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-21 (Cat no: HZ-1319) induces dose-dependent release of IFN-gamma in the NK-92 human cell line. NK-92 cells were treated with increasing concentration of recombinant IL-21 for 72 hours before supernatant collection. The supernatant was tested for IFN gamma using Proteintech’s AuthentiKine™ Human IFN-gamma ELISA Kit (KE00146). The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 0.25-1.25 ng/mL.

    Recombinant human IL-21 (Cat no: HZ-1319) induces dose-dependent release of IFN-gamma in the NK-92 human cell line. NK-92 cells were treated with increasing concentration of recombinant IL-21 for 72 hours before supernatant collection. The supernatant was tested for IFN gamma using Proteintech’s AuthentiKine™ Human IFN-gamma ELISA Kit (KE00146). The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 0.25-1.25 ng/mL.

Technical Specifications

GeneID 59067
Species Human
Expression HEK293
Activity 0.25-1.25 ng/mL
Purity >95%
Endotoxin<1 EU/μg
Accession NumberQ9HBE4
Molecular Mass 18 kDa reduced, 17 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

IL-21 is a type I, 4-α-helix bundle cytokine primarily produced by CD4+ T cells and NK cells. It is a strong activator of proliferation and antibody production in B cells but does signal to numerous other immune cells as well. Elevated IL-21 signaling can promote several autoimmune diseases including type I diabetes, systemic lupus erythematosus (SLE), and rheumatoid arthritis (RA). However, it has also been shown to have potent antitumoral activity, making it a promising candidate for immunotherapy and as an adjuvant material in CAR-T cell therapy (PMID: 22235123, 26966515, 27577879).

Synonyms

Interleukin 21, Interleukin-21, Interleukin-21 isoform, Za11, CVID11

Publications

SpeciesTitle

Immunity

Human microglia maturation is underpinned by specific gene regulatory networks

Authors - Claudia Z Han
View Article