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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-22 protein- GMP grade

EC50

0.01-0.18 ng/mL in reporter cell line

Species Reactivity

human

Purity

> 95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1325-GMP

Synonyms

IL-TIF, cytokine ZCYTO18

Validation Data Gallery

  • Purity of recombinant human IL-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-22 (HZ-1325) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 value range is 0.01-0.18 ng/mL.

    Recombinant human IL-22 (HZ-1325) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 value range is 0.01-0.18 ng/mL.

Technical Specifications

GeneID 50616
Species Human
Expression HEK293
EC50 0.01-0.18 ng/mL in reporter cell line
Purity > 95%
Endotoxin<0.1 EU/μg
Accession NumberQ9GZX6
Molecular Mass 25-40 kDa reduced and non-reduced, dimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

IL-22 is a 6-α-helix, bundle cytokine that is a member of the IL-10 family of cytokines. It is primarily produced by innate lymphoid cells (ILCS) and multiple T cell subsets including αβT, γδT, and NKT cells. Its production is regulated upstream by several different cytokines including IL-23, IL-1β, and IL-7. IL-22 binds with the IL-22 receptor, expressed on stromal and epithelial cells in a wide range of tissues, but it can also bind in circulation with the IL-22 binding protein (IL-22BP), which functions as a negative regulator. Binding with the receptor triggers several downstream pathways resulting in pro-inflammatory responses, increased mucus production, as well as increased production of anti-bacterial and anti-fungal proteins. IL-22 also plays a role in promoting wound healing and tissue regeneration in response to injury. Overexpression of IL-22 has been linked to several cancers, most notably colon cancer (PMID: 25706098, 33042126).

Synonyms

IL-TIF, cytokine ZCYTO18