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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-22 protein

Activity

0.045-0.225 ng/mL

Species Reactivity

human

Purity

> 95%

Cat No : HZ-1325

Print datasheet

Synonyms

IL-TIF, cytokine ZCYTO18

Validation Data Gallery

  • Purity of recombinant human IL-22 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-22 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-22 (Cat no: HZ-1325) induces dose-dependent release of IL-10 in the Colo 205 human cell line. Colo 205 cells were treated with increasing concentration of recombinant IL-22 for 24 hours before supernatant collection. The supernatant was tested for IL-10 via ELISA kit. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 0.045-0.225 ng/mL.

    Recombinant human IL-22 (Cat no: HZ-1325) induces dose-dependent release of IL-10 in the Colo 205 human cell line. Colo 205 cells were treated with increasing concentration of recombinant IL-22 for 24 hours before supernatant collection. The supernatant was tested for IL-10 via ELISA kit. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 0.045-0.225 ng/mL.

  • Proteintech IL-22 (Cat no: HZ-1325) demonstrates equivalent induction of IL-10 release and EC50 compared to a leading competitor. Recombinant human IL-22 induces dose-dependent release of IL-10 in the Colo 205 human cell line. Colo 205 cells were treated with increasing concentration of recombinant IL-22 for 24 hours before supernatant collection. The supernatant was tested for IL-10 via ELISA kit. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 0.045-0.225 ng/mL.

    Proteintech IL-22 (Cat no: HZ-1325) demonstrates equivalent induction of IL-10 release and EC50 compared to a leading competitor. Recombinant human IL-22 induces dose-dependent release of IL-10 in the Colo 205 human cell line. Colo 205 cells were treated with increasing concentration of recombinant IL-22 for 24 hours before supernatant collection. The supernatant was tested for IL-10 via ELISA kit. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 0.045-0.225 ng/mL.

Technical Specifications

GeneID 50616
Species Human
Expression HEK293
Activity 0.045-0.225 ng/mL
Purity > 95%
Endotoxin<1.0 EU/μg
Accession NumberQ9GZX6
Molecular Mass 25-40 kDa reduced and non-reduced, dimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

IL-22 is a 6-α-helix, bundle cytokine that is a member of the IL-10 family of cytokines. It is primarily produced by innate lymphoid cells (ILCS) and multiple T cell subsets including αβT, γδT, and NKT cells. Its production is regulated upstream by several different cytokines including IL-23, IL-1β, and IL-7. IL-22 binds with the IL-22 receptor, expressed on stromal and epithelial cells in a wide range of tissues, but it can also bind in circulation with the IL-22 binding protein (IL-22BP), which functions as a negative regulator. Binding with the receptor triggers several downstream pathways resulting in pro-inflammatory responses, increased mucus production, as well as increased production of anti-bacterial and anti-fungal proteins. IL-22 also plays a role in promoting wound healing and tissue regeneration in response to injury. Overexpression of IL-22 has been linked to several cancers, most notably colon cancer (PMID: 25706098, 33042126).

Synonyms

IL-TIF, cytokine ZCYTO18

Publications

SpeciesTitle
human

Skin Res Technol

Livin expression promotes keratinocyte release of inflammatory mediators in psoriasis

Authors - Delu Che
View Article