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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-3 protein- GMP grade

EC50

0.4-2.0 ng/mL

Specific Activity

minimally 5.00 x 105 IU/mg, typically 9.20 x 105 IU/mg

Species Reactivity

human

Purity

>95 %

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat No : HZ-1074-GMP

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Synonyms

Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, P cell stimulating factor

Validation Data Gallery

  • Purity of GMP-grade recombinant human IL-3 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of GMP-grade recombinant human IL-3 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP-grade recombinant human IL-3 (HZ-1074-GMP) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-3 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.4-2.0 ng/mL

    GMP-grade recombinant human IL-3 (HZ-1074-GMP) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-3 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.4-2.0 ng/mL

Technical Specifications

GeneID 3562
Species Human
Expression HEK293
EC50 0.4-2.0 ng/mL
Specific Activityminimally 5.00 x 105 IU/mg, typically 9.20 x 105 IU/mg
Purity >95 %
Endotoxin< 0.1 EU/μg
Accession NumberP08700
Molecular Mass 17 to 50 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1xPBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4. Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders (PMID: 24103757;2698876;2809196)

Synonyms

Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, P cell stimulating factor