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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-5 protein

Activity

0.2-0.9 ng/mL

Specific Activity

minimally 4.0 x 106 IU/mg

Species Reactivity

human

Purity

>95%

Cat No : HZ-1324

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Synonyms

IL5, IL 5, IL-5T-cell replacing factor, interleukin 5, interleukin-5, EDF, Eo-CSF, Eosinophil differentiation factor, BCDF mu, B-cell differentiation factor I, BCGFII, TRF, TRFB cell differentiation factor

Validation Data Gallery

  • Purity of recombinant human IL-5 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

    Purity of recombinant human IL-5 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

  • Recombinant human IL-5 (HZ-1324) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-5 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.2-0.9 ng/mL.

    Recombinant human IL-5 (HZ-1324) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-5 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.2-0.9 ng/mL.

Technical Specifications

GeneID 3567
Species Human
Expression HEK293
Activity 0.2-0.9 ng/mL
Specific Activityminimally 4.0 x 106 IU/mg
Purity >95%
Endotoxin<0.1 EU/µg
Accession NumberP05113
Molecular Mass 19 to 21 kDa reduced, 35 to 40 kDa non-reduced, disulfide linked homodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

IL-5 is a 4-α-helix, pleiotropic cytokine that regulates the activities of B cells, eosinophils, and basophils. It is produced mostly by CD4+ T cells, eosinophils, and basophils, and signals through its specific receptor (IL-5R) on target cells to activate downstream JAK/STAT, Btk, and Ras/ERk pathways. Due to its pleiotropic effects, IL-5 plays a role in both innate and adaptive immune responses. It is a strong promoter of eosinophil differentiation, migration, maturation, and survival. Additionally, IL-5 is also capable of stimulating activated B cells to differentiate into antibody-secreting cells. Elevated IL-5 levels are strongly correlated with eosinophilia, which is a hallmark feature of allergy, asthma, and hypersensitivity (PMID: 21986312, 22174445, 19819937, 16895557)

Synonyms

IL5, IL 5, IL-5T-cell replacing factor, interleukin 5, interleukin-5, EDF, Eo-CSF, Eosinophil differentiation factor, BCDF mu, B-cell differentiation factor I, BCGFII, TRF, TRFB cell differentiation factor