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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-8 protein

Activity

100-500 ng/mL

Species Reactivity

human

Purity

>95%

Cat No : HZ-1318

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Synonyms

CXCL8, CXCL8SCYB8, interleukin 8, IL8, AMCF-I, C-X-C motif chemokine 8, NAF, NAP1, NCF GCP1, GCP-1,TSG-1, K60, LAI, LECT, LUCT, T cell chemotactic factor, T-cell chemotactic factor, TCF, TSG1, Neutrophil-activating protein 1

Validation Data Gallery

  • Purity of recombinant human IL-8 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

    Purity of recombinant human IL-8 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

  • Recombinant human IL-8 (HZ-1318) stimulates dose-dependent proliferation of the THP-1 human monocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant IL-8 for 144 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 100-500 ng/mL.

    Recombinant human IL-8 (HZ-1318) stimulates dose-dependent proliferation of the THP-1 human monocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant IL-8 for 144 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 100-500 ng/mL.

  • Proteintech IL-8 (HZ-1318) demonstrates a 2.5-fold decrease in EC50 and a greater fold induction compared to leading competitors. Recombinant human IL-8 stimulates dose-dependent proliferation of the THP-1 human monocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant IL-8 for 144 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 100-500 ng/mL.

    Proteintech IL-8 (HZ-1318) demonstrates a 2.5-fold decrease in EC50 and a greater fold induction compared to leading competitors. Recombinant human IL-8 stimulates dose-dependent proliferation of the THP-1 human monocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant IL-8 for 144 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 100-500 ng/mL.

Technical Specifications

GeneID 3576
Species Human
Expression HEK293
Activity 100-500 ng/mL
Purity >95%
Endotoxin<0.1 EU/µg
Accession NumberP10145
Molecular Mass 11 kDa reduced, 12 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4. Gently swirl or tap vial to mix.

Background

IL-8 is a chemoattractant cytokine that signals through the CXCR1 and CXCR2 receptors to attract and activate neutrophils to sites of inflammation. It is secreted by multiple types of cells, including monocytes, neutrophils, tumor, epithelial, endothelial, and fibroblasts. IL-8 signaling activates downstream pathways that promote cell survival, proliferation, and cell invasiveness. As such, IL-8 has been linked to the promotion of angiogenesis, immunosuppressive cell recruitment, and EMT transition in several cancer types. Recent studies also indicate that IL-8 can serve as a marker for COVID-19 disease progression (PMID: 22235381, 18980965, 26548401, 33584733).

Synonyms

CXCL8, CXCL8SCYB8, interleukin 8, IL8, AMCF-I, C-X-C motif chemokine 8, NAF, NAP1, NCF GCP1, GCP-1,TSG-1, K60, LAI, LECT, LUCT, T cell chemotactic factor, T-cell chemotactic factor, TCF, TSG1, Neutrophil-activating protein 1

Publications

SpeciesTitle
human

J Ethnopharmacol

Huaier suppresses cisplatin resistance in non-small cell lung cancer by inhibiting the JNK/JUN/IL-8 signaling pathway

Authors - Haoyi Jin
View Article
human

Carcinogenesis

Lnc-PKNOX1-1 inhibits tumor progression in cutaneous malignant melanoma by regulating NF-κB/IL-8 axis

Authors - Anlan Hong
View Article
human

Nat Commun

Scar matrix drives Piezo1 mediated stromal inflammation leading to placenta accreta spectrum

Authors - Du Wenqiang
View Article