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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human VEGF-C protein

Activity

2-16 ng/mL

Species Reactivity

human

Purity

>95%

Cat No : HZ-1336

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Synonyms

FLT4 ligand, FLT4-L, Vascular endothelial growth factor related protein (VRP), VRP, VEGFC, VRPFLT4 ligand DHM

Validation Data Gallery

  • Purity of recombinant human VEGF-C was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human VEGF-C was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human VEGF-C (HZ-1336) induces dose-dependent luciferase production in a HEK293T reporter cell line. Luciferase assay production was assessed by One-Step™ luciferase assay Kit. HEK293T reporter cells were treated with increasing concentrations of recombinant VEGF-C for 18 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 2-14 ng/mL.

    Recombinant human VEGF-C (HZ-1336) induces dose-dependent luciferase production in a HEK293T reporter cell line. Luciferase assay production was assessed by One-Step™ luciferase assay Kit. HEK293T reporter cells were treated with increasing concentrations of recombinant VEGF-C for 18 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 2-14 ng/mL.

Technical Specifications

GeneID 7424
Species Human
Expression HEK293
Activity 2-16 ng/mL
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP49767
Molecular Mass 18 and 28 kDa, homodimer, glycosylated
Formulation 50 mM Acetate pH 4.5 + 250 mM NaCl
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein at 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant serum albumin (HSA). Gently swirl or tap vial to mix.

Background

N/A

Synonyms

FLT4 ligand, FLT4-L, Vascular endothelial growth factor related protein (VRP), VRP, VEGFC, VRPFLT4 ligand DHM