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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-4 protein

Activity

0.07-0.4 ng/mL

Specific Activity

minimally 5 x 106 IU/mg

Species Reactivity

human,mouse,pig

Purity

>95%

Cat No : HZ-1004

Print datasheet

Synonyms

B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra

Validation Data Gallery

  • Purity of recombinant human IL-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-4 (HZ-1004) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-4 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.07-0.4 ng/mL.

    Recombinant human IL-4 (HZ-1004) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-4 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.07-0.4 ng/mL.

Technical Specifications

GeneID 3565
Species Human
Expression HEK293
Activity 0.07-0.4 ng/mL
Specific Activityminimally 5 x 106 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP05112
Molecular Mass 15 and 21 kDa reduced, 13 and 19 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse,pig

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival (PMID: 24489573;3049907;21663408)

Synonyms

B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra

Publications

SpeciesTitle
mouse

Mol Cell

Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation

Authors - Chuanlong Wang
View Article

Small

M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation

Authors - Jie Sun
View Article
human

J Exp Med

Inherited TNFSF9 deficiency causes broad Epstein-Barr virus infection with EBV+ smooth muscle tumors.

Authors - Benjamin Fournier
View Article

Biomaterials

In situ repurposing of dendritic cells with CRISPR/Cas9-based nanomedicine to induce transplant tolerance.

Authors - Yue Zhang
View Article
mouse

ACS Appl Mater Interfaces

NIR-II Light-Actuated Nanomotors for Enhanced Photoimmunotherapy Toward Hepatocellular Carcinoma

Authors - Yichi Chen
View Article
mouse

Cell Biosci

Differential effects of PGAM5 knockout on high fat high fructose diet and methionine choline-deficient diet induced non-alcoholic steatohepatitis (NASH) in mice

Authors - Li Li
View Article