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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-7 protein

Activity

0.1-1.4 ng/mL

Specific Activity

minimally 7.00x107 IU/mg

Species Reactivity

human,mouse,rat

Purity

>95%

Cat No : HZ-1281

Print datasheet

Synonyms

IL 7, IL7, IL-7, interleukin 7

Validation Data Gallery

  • Purity of recombinant human IL-7 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-7 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-7 (HZ-1281) stimulates dose-dependent proliferation of the 2E8 murine B Lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 2E8 cells were treated with increasing concentrations of recombinant IL-7 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.1-1.4 ng/mL.

    Recombinant human IL-7 (HZ-1281) stimulates dose-dependent proliferation of the 2E8 murine B Lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 2E8 cells were treated with increasing concentrations of recombinant IL-7 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.1-1.4 ng/mL.

  • Proteintech IL-7 (HZ-1281) demonstrates 3-fold lower EC50 and equivalent induction of proliferation compared to a leading competitor. Recombinant human IL-7 stimulates dose-dependent proliferation of the 2E8 murine B lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 2E8 cells were treated with increasing concentrations of recombinant IL-7 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.1-1.4 ng/mL.

    Proteintech IL-7 (HZ-1281) demonstrates 3-fold lower EC50 and equivalent induction of proliferation compared to a leading competitor. Recombinant human IL-7 stimulates dose-dependent proliferation of the 2E8 murine B lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 2E8 cells were treated with increasing concentrations of recombinant IL-7 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.1-1.4 ng/mL.

Technical Specifications

GeneID 3574
Species Human
Expression HEK293
Activity 0.1-1.4 ng/mL
Specific Activityminimally 7.00x107 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP13232
Molecular Mass 19, 25, and 30 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse,rat

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Interleukin-7 (IL7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration (PMID: 29663382; 29655570; 29449560)

Synonyms

IL 7, IL7, IL-7, interleukin 7

Publications

SpeciesTitle

Dev Cell

Defining the identity and the niches of epithelial stem cells with highly pleiotropic multilineage potency in the human thymus

Authors - Roberta Ragazzini
View Article
human

Front Oncol

Combination of 4-1BB and DAP10 promotes proliferation and persistence of NKG2D(bbz) CAR-T cells

Authors - Cheng Wei
View Article

Biochem J

Proteomic analysis in primary T cells reveals IL-7 alters T cell receptor thresholding via CYTIP/cytohesin/LFA-1 localisation and activation

Authors - Rayner M L Queiroz
View Article
rat

Heliyon

Astragalus regulates the intestinal immune response during sepsis by mediating ILC3 proliferation through RORγt

Authors - Jin Li
View Article
human

Mol Cancer

The construction of modular universal chimeric antigen receptor T (MU-CAR-T) cells by covalent linkage of allogeneic T cells and various antibody fragments

Authors - Tao Chen
View Article