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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human Noggin protein

Activity

1.5-15 ng/mL

Species Reactivity

human,mouse

Purity

>95%

Cat No : HZ-1118

Print datasheet

Synonyms

NOG, noggin, SYM1, SYNS1

Validation Data Gallery

  • Purity of recombinant human Noggin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human Noggin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human Noggin (HZ-1118-GMP) inhibits dose-dependent induction of alkaline phosphatase production by BMP-4 in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human Noggin and 40 ng/mL of BMP-4 (HZ-1045) for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1.5-15 ng/mL.

    Recombinant human Noggin (HZ-1118-GMP) inhibits dose-dependent induction of alkaline phosphatase production by BMP-4 in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human Noggin and 40 ng/mL of BMP-4 (HZ-1045) for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1.5-15 ng/mL.

Technical Specifications

GeneID 9241
Species Human
Expression HEK293
Activity 1.5-15 ng/mL
Purity >95%
Endotoxin<1 EU/μg
Accession NumberQ13253
Molecular Mass 28 kDa reduced, 50 to 95 kDa non-reduced, homodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Noggin is an inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. It is essential for cartilage morphogenesis and joint formation. Noggin inhibits chondrocyte differentiation through its interaction with GDF5 and GDF6 (PMID: 29497255; 29167826; 29094227; 28842965).

Synonyms

NOG, noggin, SYM1, SYNS1

Publications

SpeciesTitle

Cell Stem Cell

Increased Neural Progenitor Proliferation in a hiPSC Model of Autism Induces Replication Stress-Associated Genome Instability.

Authors - Meiyan Wang
View Article
human

Nat Methods

Long-term expansion of alveolar stem cells derived from human iPS cells in organoids.

Authors - YukiYamamoto
View Article
human

Cell Stem Cell

Efficient Generation of CA3 Neurons from Human Pluripotent Stem Cells Enables Modeling of Hippocampal Connectivity In Vitro.

Authors - Anindita Sarkar
View Article
mouse

Nat Commun

Pharmacological blockade of TEAD–YAP reveals its therapeutic limitation in cancer cells

Authors - Yang Sun
View Article
human

Biomaterials

Core-shell hydrogel microfiber-expanded pluripotent stem cell-derived lung progenitors applicable to lung reconstruction in vivo.

Authors - Satoshi Ikeo
View Article
human

Curr Biol

Human-specific regulation of neural maturation identified by cross-primate transcriptomics

Authors - Sara B Linker
View Article