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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human Sonic Hedgehog (SHH) protein

Activity

Typically ≤350 ng/mL

Species Reactivity

human,mouse,rat

Purity

>90%

Cat No : HZ-1306

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Synonyms

HHG 1, HHG1, HLP3, HPE3, MCOPCB5, SHH, SMMCI, Sonic hedgehog protein, TPT, TPTPS

Validation Data Gallery

  • Purity of recombinant human SHH was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human SHH was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human SHH (HZ-1306) stimulates dose-dependent induction of alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. C3H10T1/2 cells were treated with increasing concentrations of recombinant human SHH for 120 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values are no more than 350 ng/mL.

    Recombinant human SHH (HZ-1306) stimulates dose-dependent induction of alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. C3H10T1/2 cells were treated with increasing concentrations of recombinant human SHH for 120 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values are no more than 350 ng/mL.

Technical Specifications

GeneID 6469
Species Human
Expression HEK293
Activity Typically ≤350 ng/mL
Purity >90%
Endotoxin<1.0 EU/μg
Accession NumberQ15465
Molecular Mass 19-22 kDa reduced and non-reduced, monomer, glycosylated
Formulation 10mM Tris pH 7.4 + 100mM NaCl + 1% CHAPS, See Certificate of Analysis for details
Species Reactivity human,mouse,rat

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.1 mg/mL in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

SHH, also named as HHG-1, belongs to the hedgehog family. SHH binds to the patched receptor (PTC), that in association with smoothened (SMO) activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. SHH is synthesized as a 45 kDa protein precursor that is autocatalytically cleaved to yield of 20 kDa N-terminal fragment (24-197 in the human gene sequence) knowing as being responsible for all known hedgehog biological activity and a 25 kDa C-terminal fragment that contains the auto-processing machinery (PMID:10753901).

Synonyms

HHG 1, HHG1, HLP3, HPE3, MCOPCB5, SHH, SMMCI, Sonic hedgehog protein, TPT, TPTPS

Publications

SpeciesTitle
human

Mol Biol Rep

Discovery of YS-1 as a cell line of gastric inflammatory cancer-associated fibroblasts

Authors - Satoe Numakura
View Article
rat

EBioMedicine

Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis

Authors - Shihui Xu
View Article