CoraLite® Plus 488-conjugated smooth muscle actin specific Monoclonal antibody
smooth muscle actin specific Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, pig
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1E9A11
验证数据展示
经过测试的应用
| Positive IF detected in | mouse heart tissue |
Planning an IHC experiment? We recommend our IHCeasy smooth muscle actin Ready-To-Use IHC Kit. smooth muscle actin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67735-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67735 targets smooth muscle actin specific in IF applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Applications | IF-P |
| Tested Reactivity | Human, mouse, rat, pig |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | actin, alpha 2, smooth muscle, aorta |
| Synonyms | a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, smooth muscle actin, smooth muscle actin specific |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | NM_001613 |
| Gene Symbol | ACTA2 |
| Gene ID (NCBI) | 59 |
| RRID | AB_2919532 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P62736 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 smooth muscle actin specific antibody CL488-67735 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |