CoraLite® Plus 647-conjugated smooth muscle actin specific Monoclonal antibody
smooth muscle actin specific Monoclonal Antibody for FC (Intra), IF-P
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, pig and More (1)
Applications
FC (Intra), IF-P and More (1)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1E9A11
验证数据展示
经过测试的应用
Positive IF detected in | mouse heart tissue |
Positive FC detected in | C2C12 cells |
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 2 publications below |
FC | See 1 publications below |
产品信息
CL647-67735 targets smooth muscle actin specific in IF, FC, FC (Intra) applications and shows reactivity with Human, mouse, rat, pig samples.
Tested Applications | FC (Intra), IF-P |
Cited Applications | IF, FC |
Tested Reactivity | Human, mouse, rat, pig |
Cited Reactivity | human, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | actin, alpha 2, smooth muscle, aorta |
Synonyms | a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, smooth muscle actin, smooth muscle actin specific |
Calculated Molecular Weight | 42 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | NM_001613 |
Gene Symbol | ACTA2 |
Gene ID (NCBI) | 59 |
RRID | AB_2920302 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P62736 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 647 smooth muscle actin specific antibody CL647-67735 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Med Virol Molecular and immune signatures, and pathological trajectories of fatal COVID-19 lungs defined by in situ spatial single-cell transcriptome analysis | ||
Nat Commun FAM3A reshapes VSMC fate specification in abdominal aortic aneurysm by regulating KLF4 ubiquitination |