WB result of CDK6 antibody (14052-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK6 transfected HeLa cells.
Various lysates were subjected to SDS PAGE followed by western blot with 14052-1-AP (CDK6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP result of anti-CDK6 (IP:14052-1-AP, 4ug; Detection:14052-1-AP 1:1000) with Jurkat cells lysate 2880 ug.
MOLT-4 cells were subjected to SDS PAGE followed by western blot with 14052-1-AP (CDK6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14052-1-AP (CDK6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK6 (14052-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).