1X10^6 PHA-treated (day 3) human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human ALCAM (CL555-65223, Clone:3A6) (red), or unstained (blue). The black dashed line indicates unactivated PBMCs surface stained with 5 ul CoraLite® Plus 555 Anti-Human ALCAM (CL555-65223, Clone:3A6). Cells were not fixed. Monocytes were gated.