Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20785-1-AP (GLAST antibody at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20785-1-AP (GLAST antibody at dilution of 1:50 (under 40x lens).
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20785-1-AP (GLAST antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP result of anti-GLAST (IP:20785-1-AP, 4ug; Detection:20785-1-AP 1:500) with mouse brain tissue lysate 3000ug.
Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using GLAST antibody (20785-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using GLAST antibody (20785-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human GLAST (20785-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).