Various lysates were subjected to SDS PAGE followed by western blot with 10883-1-AP (P16-INK4A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB result of p16-INK4A antibody (10883-1-AP, 1:2,000) with si-Control and si-p16 transfected HEK-293 cells.
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10883-1-AP (P16-INK4A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-P16-INK4A(IP:10883-1-AP, 4ug; Detection:10883-1-AP 1:4000) with HEK-293 cells lysate 1400 ug.
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10883-1-AP (P16-INK4A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human P16-INK4A (10883-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).