验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells, NIH/3T3 cells, mouse liver tissue, mouse kidney tissue, mouse brain tissue, rat liver tissue, rat kidney tissue |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | mouse brain tissue, human kidney tissue, human liver tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells, NIH3T3 cells, MDCK cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17711-1-AP targets ARL13B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human, mouse, rat, canine |
| Cited Reactivity | human, mouse, rat, pig, canine, monkey, chicken, zebrafish, sheep, xenopus |
| Immunogen | ARL13B fusion protein Ag12015 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ADP-ribosylation factor-like 13B |
| Synonyms | ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 40-48 kDa, 60 kDa |
| GenBank Accession Number | BC094725 |
| Gene Symbol | ARL13B |
| Gene ID (NCBI) | 200894 |
| RRID | AB_2060867 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q3SXY8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARL13B antibody 17711-1-AP | Download protocol |
| IHC protocol for ARL13B antibody 17711-1-AP | Download protocol |
| IF protocol for ARL13B antibody 17711-1-AP | Download protocol |
| IP protocol for ARL13B antibody 17711-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Guided self-organization and cortical plate formation in human brain organoids. | ||
Nature Quantitative lineage analysis identifies a hepato-pancreato-biliary progenitor niche. | ||
Science Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium. | ||
Nature A slow-cycling LGR5 tumour population mediates basal cell carcinoma relapse after therapy. | ||
Nature Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size. |
