验证数据展示
经过测试的应用
| Positive IF/ICC detected in | hTERT-RPE1 cells, MDCK cells |
For other applications, we recommend the unconjugated version of this antibody, 17711-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 2 publications below |
产品信息
CL488-17711 targets ARL13B in IF/ICC applications and shows reactivity with human, mouse, rat, Canine samples.
| Tested Applications | IF/ICC Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat, Canine |
| Cited Reactivity | human, mouse |
| Immunogen | ARL13B fusion protein Ag12015 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ADP-ribosylation factor-like 13B |
| Synonyms | ARL13B, ARL2 like protein 1, ARL2L1, ARL2-like protein 1, ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1 |
| Calculated Molecular Weight | 48 kDa |
| GenBank Accession Number | BC094725 |
| Gene Symbol | ARL13B |
| Gene ID (NCBI) | 200894 |
| RRID | AB_2883085 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q3SXY8 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). CoraLite488-Conjugated ARL13B antibody can be used to mark the cilia(green). Ex: 488nm, Em: 515nm
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ARL13B antibody CL488-17711 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Oncol Interferon regulatory factor 4 modulates epigenetic silencing and cancer-critical pathways in melanoma cells | ||
Neurosci Res Phenotypic characterization of Cdkl5-knockdown neurons establishes elongated cilia as a functional assay for CDKL5 Deficiency Disorder. |