• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of BxPC-3 using 16549-1-AP

    WB analysis of BxPC-3 using 16549-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of BxPC-3 using 16549-1-AP

    IF Staining of BxPC-3 using 16549-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 16549-1-AP (CCKBR-specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CCKBR-specific Polyclonal antibody

CCKBR-specific Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16549-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA and More (2)

别名

CCKBR, CCK2-R, CCK2R, CCK2 R, CCK BR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inBxPC-3 cells, human stomach tissue, SGC-7901 cells
Positive IF/ICC detected inBxPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16549-1-AP targets CCKBR-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cholecystokinin B receptor
别名 CCKBR, CCK2-R, CCK2R, CCK2 R, CCK BR
计算分子量 56 kDa
观测分子量 64 kDa, 50 kDa
GenBank蛋白编号NM_176875
基因名称 CCKBR
Gene ID (NCBI) 887
RRIDAB_2070508
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP32239
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cholecystokinin B receptor (CCKBR) is a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors.

实验方案

Product Specific Protocols
WB protocol for CCKBR-specific antibody 16549-1-APDownload protocol
IF protocol for CCKBR-specific antibody 16549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Mol Pharm

Pseudomonas Exotoxin A-Based Immunotherapy Targeting CCK2R-Expressing Colorectal Malignancies: An In Vitro and In Vivo Evaluation.

Authors - Jiang Chang
View Article
humanIF

J Cancer Res Clin Oncol

Cholecystokinin type 2 receptor in colorectal cancer: diagnostic and therapeutic target.

Authors - Jiang Chang
View Article