CHCHD3 Polyclonal antibody
CHCHD3 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, human placenta tissue, A431 cells, mouse liver tissue, HeLa cells, Jurkat cells, NIH/3T3 cells, NIH/3T3 cells. |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human liver tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:4000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25625-1-AP targets CHCHD3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | CHCHD3 fusion protein Ag22579 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | coiled-coil-helix-coiled-coil-helix domain containing 3 |
Synonyms | CHCHD3, MIC19, MINOS3 |
Calculated Molecular Weight | 227 aa, 26 kDa |
Observed Molecular Weight | 26 kDa |
GenBank Accession Number | BC014839 |
Gene Symbol | CHCHD3 |
Gene ID (NCBI) | 54927 |
RRID | AB_2687533 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NX63 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CHCHD3 antibody 25625-1-AP | Download protocol |
IHC protocol for CHCHD3 antibody 25625-1-AP | Download protocol |
IF protocol for CHCHD3 antibody 25625-1-AP | Download protocol |
IP protocol for CHCHD3 antibody 25625-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Acta Neuropathol Mitochondria, ER, and nuclear membrane defects reveal early mechanisms for upper motor neuron vulnerability with respect to TDP-43 pathology. | ||
Mol Cell Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria. | ||
Nat Commun Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA | ||
EMBO Rep Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner. | ||
Cell Rep Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control. | ||
Sci Rep Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure. |