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COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal antibody
COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF-P, ELISA and More (2)
Conjugate
Unconjugated
143
CloneNo.
3G2B9
验证数据展示
经过测试的应用
Positive WB detected in | RAW 264.7 cells, LPS treated RAW 264.7 cells, LPS treated HeLa cells, HEK-293 cells |
Positive IHC detected in | human cervical cancer tissue, human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse lung tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66351-1-Ig targets COX2/ Cyclooxygenase 2/ PTGS2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag24721 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) |
Synonyms | COX 2, COX2, COX2/ Cyclooxygenase 2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2 |
Calculated Molecular Weight | 604 aa, 68 kDa |
Observed Molecular Weight | 66-70 kDa |
GenBank Accession Number | BC013734 |
Gene Symbol | COX2/PTGS2 |
Gene ID (NCBI) | 5743 |
RRID | AB_2881731 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P35354 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-Ig | Download protocol |
IHC protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-Ig | Download protocol |
IF protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 66351-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Metab Cooperation between COA6 and SCO2 in COX2 maturation during cytochrome c oxidase assembly links two mitochondrial cardiomyopathies. | ||
ACS Nano Engineered Extracellular Vesicle-Delivered CRISPR/Cas9 for Radiotherapy Sensitization of Glioblastoma | ||
Redox Biol The deubiquitinase USP11 ameliorates intervertebral disc degeneration by regulating oxidative stress-induced ferroptosis via deubiquitinating and stabilizing Sirt3 | ||
J Nanobiotechnology Injectable photocrosslinking spherical hydrogel-encapsulated targeting peptide-modified engineered exosomes for osteoarthritis therapy | ||
J Nanobiotechnology Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression | ||
ACS Appl Mater Interfaces A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells |