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CX3CL1 Monoclonal antibody

CX3CL1 Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC, IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2F5D5

Cat No : 60339-1-Ig

Print datasheet

Synonyms

ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3CL1, CXC3, CXC3C, FKN, fractalkine, neurotactin, NTN, NTT, SCYD1, Small inducible cytokine D1

验证数据展示

  • WB analysis of PC-3 using 60339-1-Ig

    WB analysis of PC-3 using 60339-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60339-1-Ig (CX3CL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 60339-1-Ig

    IHC staining of human brain using 60339-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60339-1-Ig (CX3CL1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human brain using 60339-1-Ig

    IHC staining of human brain using 60339-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60339-1-Ig (CX3CL1 Antibody) at dilution of 1:200 (under 40x lens).

  • FC experiment of PC-3 using 60339-1-Ig

    FC experiment of PC-3 using 60339-1-Ig

    1X10^6 PC-3 cells were stained with 0.2 ug Anti-Human CX3CL1 (60339-1-Ig, Clone: 2F5D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug Mouse IgG1 Isotype Control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH.

经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inhuman brain tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inPC-3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

60339-1-Ig targets CX3CL1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IHC, WB,ELISA
Cited ApplicationsIF
Tested Reactivity human
Cited Reactivityhuman
Immunogen CX3CL1 fusion protein Ag0161 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chemokine (C-X3-C motif) ligand 1
Synonyms ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3CL1, CXC3, CXC3C, FKN, fractalkine, neurotactin, NTN, NTT, SCYD1, Small inducible cytokine D1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC001163
Gene Symbol CX3CL1
Gene ID (NCBI) 6376
RRIDAB_2881448
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP78423
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CX3CL1 antibody 60339-1-IgDownload protocol
IHC protocol for CX3CL1 antibody 60339-1-IgDownload protocol
FC protocol for CX3CL1 antibody 60339-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

JCI Insight

Single-cell transcriptome analysis and protein profiling reveal broad immune system activation in IgG4-related disease

Authors - Chenyang Lu
View Article